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mrrby  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mrrby
    Mrrby, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mrrby/Sucrose/pm41734064-264-176-178
    Average 98 stars, based on 1 article reviews
    mrrby - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Biomarker Discovery:

    Article Title: Microbial short-chain fatty acids modulate CD8 + T cell responses and improve adoptive immunotherapy for cancer
    Article Snippet: All antibodies used for cytometry are from commercial sources and were purchased from Biolegend, eBioscience, BD Pharmingen/ Bioscience, Invitrogen or Miltenyi Biotech. .. Anti-mouse: CD3, clone 145-2C11 (Cat# 100312 - Biolegend, Cat# 100306 - Biolegend, Cat# 100302 - Biolegend) CD28, clone 37.51 (Cat# 102116 - Biolegend) CD4, clone RM4-5 (Cat# 100510 - Biolegend, Cat# 100538 - Biolegend, Cat# 560468 - BD) CD8, clone 53-6.7 (Cat# 100706 - Biolegend, Cat# 100712 - Biolegend, Cat# 560776 - BD) CD45.1, clone A20 (Cat# 110726 - Biolegend) CD25, clone PC61.5 (Cat# 102012 - 110726, Cat# 102016 - 110726) CD19, clone 1D3/CD19 (Cat# 152406 - Biolegend) IFN-gamma, clone XMG1.2 (Cat# 505810 - Biolegend, Cat# 16-7311-85 - Invitrogen) TNF-alpha, clone MP6-XT22 (Cat# 506304 - Biolegend, Cat# Biolegend) Granzyme B, clone QA16A02 (Cat# 372208 - Biolegend) Eomes, clone Dan11mag, (Cat# 48-4875-82 - eBioscience) 3 nature research | rep orting sum m ary April2020 Validation T-bet, clone eBio4B10 (Cat# 12-5825-82 - eBioscience) phospho-STAT5 (Tyr694), clone SRBCZX (Cat# 12-9010-42 - eBioscience) phospho-mTOR (ser2448), clone MRRBY (Cat# 12-9718-42 - eBioscience) phospho-S6 (Ser235/236), clone cupk43k (Cat# 17-9007-42 - eBioscience) Anti-human: IFN-gamma, clone 4S.B3 (Cat# 502512 - Biolegend) Granzyme B, clone QA16A02 (Cat# 372208 - Biolegend) CD25, clone M-A251 (Cat# 356106 - Biolegend) CD69, clone FN50 (Cat# 310914 - Biolegend) CD8, clone SK1 (Cat# 561618 - BD) For the analysis of secreted cytokines, following commercial kits were used: BioLegend ELISA MAX Deluxe Set Human IFN-y (Cat# 430104) BioLegend ELISA MAX Deluxe Set Human IL-2 (Cat# 431804) BioLegend ELISA MAX Deluxe Set Human TNF-a (Cat# 430204) Invitrogen Murine TNF-alpha ELISA Kit (Cat# 88-7324-76) Biolegend Max Standard Set Mouse IL-2 (Cat# 431001) No customized antibodies were used. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Microbial short-chain fatty acids modulate CD8 + T cell responses and improve adoptive immunotherapy for cancer
    Article Snippet: All antibodies used for cytometry are from commercial sources and were purchased from Biolegend, eBioscience, BD Pharmingen/ Bioscience, Invitrogen or Miltenyi Biotech. .. Anti-mouse: CD3, clone 145-2C11 (Cat# 100312 - Biolegend, Cat# 100306 - Biolegend, Cat# 100302 - Biolegend) CD28, clone 37.51 (Cat# 102116 - Biolegend) CD4, clone RM4-5 (Cat# 100510 - Biolegend, Cat# 100538 - Biolegend, Cat# 560468 - BD) CD8, clone 53-6.7 (Cat# 100706 - Biolegend, Cat# 100712 - Biolegend, Cat# 560776 - BD) CD45.1, clone A20 (Cat# 110726 - Biolegend) CD25, clone PC61.5 (Cat# 102012 - 110726, Cat# 102016 - 110726) CD19, clone 1D3/CD19 (Cat# 152406 - Biolegend) IFN-gamma, clone XMG1.2 (Cat# 505810 - Biolegend, Cat# 16-7311-85 - Invitrogen) TNF-alpha, clone MP6-XT22 (Cat# 506304 - Biolegend, Cat# Biolegend) Granzyme B, clone QA16A02 (Cat# 372208 - Biolegend) Eomes, clone Dan11mag, (Cat# 48-4875-82 - eBioscience) 3 nature research | rep orting sum m ary April2020 Validation T-bet, clone eBio4B10 (Cat# 12-5825-82 - eBioscience) phospho-STAT5 (Tyr694), clone SRBCZX (Cat# 12-9010-42 - eBioscience) phospho-mTOR (ser2448), clone MRRBY (Cat# 12-9718-42 - eBioscience) phospho-S6 (Ser235/236), clone cupk43k (Cat# 17-9007-42 - eBioscience) Anti-human: IFN-gamma, clone 4S.B3 (Cat# 502512 - Biolegend) Granzyme B, clone QA16A02 (Cat# 372208 - Biolegend) CD25, clone M-A251 (Cat# 356106 - Biolegend) CD69, clone FN50 (Cat# 310914 - Biolegend) CD8, clone SK1 (Cat# 561618 - BD) For the analysis of secreted cytokines, following commercial kits were used: BioLegend ELISA MAX Deluxe Set Human IFN-y (Cat# 430104) BioLegend ELISA MAX Deluxe Set Human IL-2 (Cat# 431804) BioLegend ELISA MAX Deluxe Set Human TNF-a (Cat# 430204) Invitrogen Murine TNF-alpha ELISA Kit (Cat# 88-7324-76) Biolegend Max Standard Set Mouse IL-2 (Cat# 431001) No customized antibodies were used. ..

    Recombinant:

    Article Title: Glutathione is critical for NK cell-mediated immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 7-ADD (1:100) Thermo Fisher Scientific # A1310 CD107a-PE Clone 1D4B (1:200) Thermo Fisher Scientific #15248639 CD11b-Pacific Blue Clone M1/70 (1:200) Biolegend #101224 CD11b-APC-eFluor780 Clone M1/70 (1:200) Thermo Fisher Scientific #15391420 CD19-PE Clone 1D3 (1:200) Biolegend #152407 CD19-APC-eFluor 780 Clone 1D3 (1:200) Thermo Fisher Scientific #15331430 CD27-FITC Clone LG.3A10 (1:200) Biolegend #124207 CD3ε-APC/Cy7 Clone 145-2C11 (1:200) Biolegend #100330 CD3ε-APC-eFluor 780 Clone 17A2 (1:200) Thermo Fisher Scientific #15311420 CD336 (TIM-3)-BV785 Clone RMT3-23 (1:200) Biolegend #119725 CD44-PE/Cy7 Clone IM7 (1:200) Thermo Fisher Scientific #15370920 CD49b-BV785 Clone HMα2 (1:200) Biolegend #103518 CD4-BV785 Clone GK1.5 1:200) Biolegend #100453 CD5-APC-eFluor 780 Clone 53–7.3 (1:200) Thermo Fisher Scientific #15539506 CD62L-Pacific Blue Clone MEL-14 (1:200) Biolegend #104424 CD8α-BUV-395 Clone 53–6.7 (1:200) BD Biosciences #563786 Granzyme B-APC Clone QA16A02 (1:200) Biolegend #372203 IFN-γ-APC Clone XMG1.2 (1:200) Biolegend #505810 Ki67-PE Clone 11F6 (1:200) Biolegend #151209 Ly6G/Ly6C-APC-eFLuor780 Clone RB6-8C5 (1:200) Thermo Fisher Scientific #15391660 NK1.1-APC Clone PK136 (1:200) Biolegend #108710 NK1.1-PE Clone PK136 (1:200) Biolegend #108708 NKG2D-FITC Clone C7 (1:200) Biolegend #115711 PD-1-BV605 Clone 29F.1A12 (1.200) Biolegend #135220 pmTOR S2448-eFluor450 Clone MRRBY (1:200) Thermo Fisher Scientific #15549836 pS6 S235/S236-APC Clone cupk43k (1:200) Thermo Fisher Scientific #15528216 Puromycin-AF488 Clone 12D10 (1:200) Merck/Sigma Aldrich #MABE343-AF488 p-STAT5 (Y694)-FITC Clone [Stat5Y694-G11] (1:200) Abcam #ab278791 TER119-APC-eFluor780 Clone TER-119 (1:200) Thermo Fisher Scientific #15371660 TIGIT (Vstm3)-PE Clone 1G9 (1:200) Biolegend #142104 Chemicals, peptides, and recombinant proteins N-Acetyl-L-cysteine (NAC) Sigma-Aldrich #A7250-100G L-Buthionine-sulfoximine Sigma-Aldrich #B2515 PBS (1X) without Ca++, Mg++, 500mL Westburg #BE17-516F FBS Superior Lot: 0193F Biochrom GmbH #S0615 Penicillin-Streptomycin (10,000 U/mL) Gibco #11548876 L-Glutamine Westburg #BE17-605E 2-Mercaptoethanol Gibco #11508916 Glutamax Thermo Fisher Scientific #35050061 Gibco TM Sodium Pyruvate Thermo Fisher Scientific #12539059 EDTA Sigma-Aldrich #EDS-100G Bovine Serum Albumin (BSA) Sigma-Aldrich #A9647-500G RPMI 1640 (without L-Glutamine) Westburg #BE12-167F IMDM with HEPES and L-Glutamine Westburg #BE12-722F (Continued on next page) 16 Cell Reports 45, 116986, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MycoStrip_Mycoplasma detection kit Invivogen #rep-mys-10 IL-15, mouse Peprotech #210-15 Formaldehyde Sigma-Aldrich #252549-1L Saponin Sigma-Aldrich #S4521-25G Monobromobimane (mBBr) Thermo Fisher Scientific #M1378 H2DCFDA (1:2000) Thermo Fisher Scientific #D399 Hoechst 33342, Tocris Bioscience TM Thermo Fisher Scientific #30002028 H-2D(b) LCMV GP var C41M 33–41 KAVYNFATM NIH Tetramer Facility N/A H-2D(b) LCMV NP 396–404 FQPQNGQFI NIH Tetramer Facility N/A I-A(b) LCMV GP 66–77 DIYKGVYQFKSV NIH Tetramer Facility N/A GP 33-41 KAVYNFAT GenScript (Hong Kong) N/A NP 396-404 FQPQNGQFI GenScript (Hong Kong) N/A GP 66-77 DIYKGVYQFKSV GenScript (Hong Kong) N/A MitoSOX TM Red Mitochondrial Superoxide Indicator (1:5000) Thermo Fisher Scientific #M36008 MitoTracker TM Deep Red FM (1:10.000) Thermo Fisher Scientific #M22426 MitoTracker Green FM (1:100.000) Thermo Fisher Scientific #M7514 DAPI (1:2000) Thermo Fisher Scientific #D1306 Zombie NIR TM Fixable Viability Kit (1:1000) Biolegend #423106 Zombie Green TM Fixable Viability Kit (1:1000) Biolegend #423112 Oligomycin A Sigma-Aldrich #75351-5MG FCCP Sigma-Aldrich #C2920-10MG Antimycin A Sigma-Aldrich #A8674-25MG Rotenone Sigma-Aldrich #R8875-1G Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich #P8139-1MG Streptavidin APC Biolegend #405207 Calcium Ionophore A23187 Sigma-Aldrich #C7522-1MG GolgiPlug TM (Protein Transport Inhibitor) BD Biosciences #555029 RNase Thermo Fisher Scientific #R1253 Critical commercial assays NK Cell Isolation Kit, mouse Miltenyi Biotec #130-115-818 Tumor Dissociation Kit, mouse Miltenyi Biotec #130-096-730 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific #15151976 BD Cytofix/Cytoperm Fixation/Permeabilization kit BD Biosciences #554714 IFN gamma Mouse Uncoated ELISA Invitrogen TM #15501107 GSH/GSSG-Glo TM Assay Promega #V6612 CellTiter-Glo® Promega #G7571 RNeasy Micro Kit Qiagen #74004 SPOTCHEM II LIVER-1 ARKRAY #77182 PTK analysis kit Pamgene #86402 STK analysis kit Pamgene #87102 Experimental models: Organisms/strains Gclc fl/fl : B6 Mak et al. 9 N/A NCR1-Cre: B6 Narni-Mancinelli at al. 34 N/A Lymphocytic choriomeningitis virus WE – N/A B16-F10 – N/A MC-38 – N/A Deposited data RNA-Seq This manuscript GEO: GSE290323 (Continued on next page) Cell Reports 45, 116986, March 24, 2026 17



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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: The direct and indirect inhibition of proinflammatory adipose tissue macrophages by acarbose in diet-induced obesity

    doi: 10.1016/j.xcrm.2024.101883

    Figure Lengend Snippet:

    Article Snippet: Phospho-mTOR Monoclonal Antibody, PE (clone MRRBY) , Thermo Fisher Scientific , Cat#: 12-9718-42; RRID: AB_2572724.

    Techniques: Recombinant, Staining, SYBR Green Assay, Isolation, Sequencing, Software, Gentle, Control